Assuming you're running either XP or W2K...
How To Restore the System/Boot Drive Letter in Windows
http://support.microsoft.com/default...b;en-us;223188
This article describes how to change the system or boot drive letter in Windows. For the most part, this is not recommended, especially if the drive letter is the same as when Windows was installed. The only time that you may want to do this is when the drive letters get changed without any user intervention. This may happen when you break a mirror volume or there is a drive configuration change. This should be a rare occurrence and you should change the drive letters back to match the initial installation.