Just using the online banking service now and it says my balance is zero but if i look at transactions/statements its got an entry for the 28th saying i put the cheque in and the amount which was correct.
Anyway i went to a cash machine the following day(friday) and it said balance

my balance here) and then amount available today: £0.00. so doesnt look like i can draw some out unless you mean actually going into barclays and getting it from the counter?